一种简单、有效的提取根瘤菌总RNA的方法

介绍了一种适合于根瘤菌及其它革兰氏阴性细菌的RNA提取方法。它具有简便、快速等特点,且所提取的RNA样品质量较高,可直接用于RT-PCR合成、Northern杂交等分子生物学操作。该方法具有实用性强、重复性好的特点。提取的RNA无DNA等污染物,并且其产量、纯度完全能满足分子克隆和基因表达研究的需要。该方法简单、快速、纯度高、完整性强,方便有效。     

Site Productivity, the Key to Crop Productivity

If we call a significant yield increase in single crops a ‘green revolution’, then the first green revolution took place about 10 000–12 000 years ago, when humans started to cultivate land. This was also the beginning of civilization. Since then, humans have increasingly transformed the land and natural vegetation and have risen to be the main creators of the biogeosphere. Today, there is hardly any ecosystem around the globe that has not been influenced by humans. It was only in 1930 that the world population reached 2 billion, and since then it has increased to 6 billion in the year 2000. Because of this rapid increase, the demand for food, feed and industrial crops has grown enormously. Half of the 1.5 billion ha of arable land – 18 % of the biologically productive land area of the earth – was first cultivated only in the 20th Century, and mostly forest was sacrificed to meet this requirement. The second green revolution started only in the late 1960s when high‐yielding varieties of wheat and rice were designed to overcome the predicted hunger crisis. Great achievements were made, especially in relation to irrigated agriculture, while rain‐fed farming was hardly affected by this revolution. World agriculture today faces two major constraints to which not enough attention is paid by scientists and decision makers. First, we increasingly restrict our food basis to a limited number of plant species. Today, 65 % of the world's arable land is reserved for only 21 annual crops. Even more worrying is the fact that 60 % of our food energy and protein comes from only three cereals – wheat, rice and corn. Designing ‘functional’ food using gene technology to improve food quality, for example in rice, will speed up this process of constriction since diversified ingestion is no longer necessary to meet the daily required balance of food types. Secondly, arable land resources are under‐utilized because of poor management. Yields of rice, for example, are below the world average of 3.8 t ha^?1 (1998) in 70 countries. If those countries achieved only the average level for their continent, for example 2.2 t ha^?1 in Africa, world rice production could be increased by 17 %. There is a need to invest in better management of arable land to prevent further loss in productivity and simultaneously to investigate under‐explored plants to broaden our future food basis.     

基于主成分分析法的城市化发展限制因素分析

采用1991-2004年与城市化相关的统计数据作为研究的基础数据,运用因素分析实证研究法,分析我国当前城市化发展过程中的限制因素,为我国的城市化发展道路提供方向.研究结果表明：城市基础设施落后是制约城市化发展的主要限制因素,在今后的城市化道路上应着重提高城市的质量走集约型城市的路线.     

长白山红松阔叶林鸟类多样性季节格局的初步研究

采用时间序列分析方法,研究了长白山红松阔叶林鸟类多样性的季节格局。结果表明Shan non多样性指数季节格局表现为春秋高、冬夏低的双峰型特点,并与物种、数量和优势度之间有极显著的相关关系;群落各指数季节间的显著差异主要表现在冬季与其他各季之间;各月前两个优势种的比例在多样性格局构成中具有非常重要的作用;5月前和9月后,气温对Shannon指数格局的影响极为显著(r=0 9209,P<0 01)。     

总可捕量制度在我国的可行性分析

本文在论述总可捕量制度的定义、实施总可捕量制度需要具备的必要条件等内容的基础上，指出我国实施总可捕量制度的必然趋势及存在的困难。并提出了我国实施总可捕量制度的四点建议和设想：（1）大力实施减船措施，（2）加强渔业资源的调查与研究，（3）加强渔政执法，重视监督管理，（4）由点到面逐步实施TAC制度。     

Feeding of Atlantic salmon, Salmo salar L., parr in the subarctic River Teno and three tributaries in northernmost Finland

Abstract— Available food and feeding of Atlantic salmon parr were studied in different parts of a subarctic river in northernmost Finland, the River Teno: in the large main stem, in a major tributary influenced by lakes, and in two minor tributaries. The highest density of benthic organisms was found in an outlet of a lake, but drifting organisms were the most abundant in the small tributaries. Migrant parr that move to the small tributaries from the main stem have better food resources in their new habitat. Plecoptera nymphs were the most important prey for salmon parr early in the spring. Later in the summer dipterans were usually the dominant prey group. In September the importance of Plecoptera was emphasized in the small tributaries and the lake outlet, and that of Trichoptera in other sites. Drifting prey was used more by large than by small parr. The utilization of drifting food appeared to be related to the water temperature rather than to the availability of the drift.     

Expression of hypoxia-inducible factor 1 and neuroglobin in piglet cortex during deep hypothermic circulatory arrest

AIM: To observe the expression of hypoxia-inducible factor 1 (HIF-1) and neuroglobin (NGB) in piglet cortex during deep hypothermic circulatory arrest. METHODS: Wuzhishan piglets were randomly assigned to cardiopulmonary bypass group (CPB group), 40 min of circulatory arrest (CA) at 18 ℃ without cerebral perfusion (DHCA group) or with selective antegrade cerebral perfusion (SACP group). After 180 min of reperfusion, cortical tissue was harvested for determining HIF-1α and NGB expression by HE staining, Western blot and real-time PCR. RESULTS: Severer cerebral injury was observed in DHCA group than that in SACP group. After 180 min of reperfusion, HIF-1α protein and mRNA levels were significantly higher in DHCA group than those in CPB group (P<0.05). Accordingly, SACP animal had higher levels of HIF-1α protein and mRNA than those in DHCA group (P<0.05). Simultaneously, higher NGB protein and mRNA levels were found in DHCA group than those in CPB group after 180 min of reperfusion (P<0.05). The SACP animal had higher levels of NGB protein and mRNA than those in DHCA group (P<0.05). CONCLUSION: Up-regulation of HIF-1 and NGB are involved in the mechanism against cerebral injury resulting from DHCA in the cortex and possibly a part of cerebral protective effect of SACP.     

辣椒全基因组WRKY转录因子的分析

基于已公布的辣椒全基因组数据,利用生物信息学方法对辣椒WRKY转录因子家族进行全面鉴定和系统命名,并在此基础上对基因分类、染色体定位、系统进化关系和结构域序列保守性进行了研究。结果表明:辣椒CaWRKY家族包含71个基因,根据WRKY结构域的数量及锌指结构的特征可将其分为GroupⅠ、GroupⅡ和GroupⅢ等3大类,GroupⅡ又可分为Ⅱ(a)、Ⅱ(b)、Ⅱ(c)、Ⅱ(d)和Ⅱ(e)等5个亚类。辣椒12条染色体上均有WRKY转录因子分布,其中第1号染色体上分布最多,共有10个,第4号染色体上分布最少,仅有2个。辣椒每类/亚类WRKY几乎含有相同的保守基序。辣椒WRKY编码的蛋白在132~869个氨基酸范围内,平均氨基酸数量为373个。     

Protective effect of BNDF on vascular endothelial cells with H2O2-induced oxidative injury

AIM: To study the protective effect of brain-derived neurotrophic factor (BDNF) on vascular endothelial cells with H₂O₂-induced oxidative injury. METHODS: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro, and the oxidation injury model of HUVECs was established by treatment with H₂O₂. The oxidatively injured HUVECs were cultured with different concentrations (1, 10 and 100 μg/L) of BDNF. At the same time, the control group (no injury), PBS treatment after H₂O₂ injury group and TrkB inhibitor group (with 100 μg/L BDNF and 1: 1 000 TrkB inhibitor) were also set up. The viability of the HUVECs was detected by MTT assay. The levels of LDH, MDA, SOD and GSH were measured. The releases of NO, ET-1 and ICAM-1 were analyzed by ELISA. The changes of ROS production and cell apoptosis were evaluated by flow cytometry. The protein levels of TrkB, p-TrkB, cleaved caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with uninjured control group, in H₂O₂ oxidative injury plus PBS treatment group, the viability of the cells was decreased significantly, the LDH and MDA levels were increased significantly and the activities of SOD and GSH were decreased significantly. The NO secretion was decreased, and the ET-1 and ICAM-1 concentrations were increased significantly. The ROS content and apoptotic rate were increased significantly. The protein levels of cleaved caspase-3 and Bax were increased but Bcl-2 protein expression was decreased significantly. Compared with PBS treatment group, in H₂O₂-injured HUVECs treated with different concentrations of BDNF, the cell viability was gradually increased, the LDH and MDA levels were decreased and the activities of SOD and GSH were increased gradually. The secretion of NO was increased but ET-1 and ICAM-1 were decreased gradually. The ROS content and apoptotic rate were decreased significantly. The TrkB and p-TrkB levels were significantly increased significantly, the protein expression of cleaved-caspase 3 and Bax was decreased gradually and the Bcl-2 protein expression increased gradually. The role of BDNF was inhibited by TrkB inhibitor. CONCLUSION: BDNF protects HUVECs from oxidative injury by binding with TrkB to activate the BDNF-TrkB signaling pathways.     

对我国食品安全问题的思考

介绍了食品生产、流通以及消费过程中存在的导致食品不安全的因素,论述了食品安全问题的重要性和特殊性,针对这些问题提出了解决食品安全的对策建议.